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SERPICO Research team

Space-timE RePresentation, Imaging and cellular dynamics of molecular COmplexes

Team presentation

Multidimensional and multimodal light microscopy combined with GFP (Green Fluorescence Protein) tagging has taken a prominent role in life science research due to its ability to study in vitro and in vivo biomolecules in the cell compartments and cell domains.

The main objective of SERPICO team is to decipher the dynamic coordination and organization of molecular complexes at the single cell level. Our first aim is to foster dedicated technological and methodological developments to build an integrated imaging approach that bridges the resolution gaps between the molecule and the whole cell, including their temporal behavior. While we will focus on particular biological models of endo-membrane biogenesis and trafficking in the endosomal-recycling pathway of specialized cells, most of results and developments will apply in different fields of cell and integrative biology. A global and pluridisciplinary (applied mathematics, computer science, biology, physics ...) approach is necessary to identify the molecular processes resulting in pathological situations (cancer, degenerative diseases ...), as well as to validate future therapeutic agents.

In collaboration with UMR 144 CNRS Institut Curie ("Subcellular Structure and Cellular Dynamics" Unit and PICT (Cell and Tissue Imaging Facilities) -IBiSA), SERPICO team provides computational methods and mathematical models to automatically extract, organize and model information present in temporal series of images as they are obtained in multidimensional light microscopy.

Research themes

To link accurately all data information rising from different imaging modalities, further developments in image analysis, multiscale modeling and statistics are required. We address the following themes :
  • Image superresolution and image denoising to preserve cell integrity (photo-toxicity versus exposure time)
  • Information extraction from images and videos in multidimensional microscopy for molecular interaction analysis
  • Spatio-temporal organization modeling of molecular species and multiscale architectures
  • Computational simulation and modeling of membrane transport at different spatial and temporal scales

International and industrial relations

  • UMR 144 CNRS Institut Curie et PICT-IBiSA
  • UMR 6026 CNRS Université Rennes 1
  • INRA Unité MIA Jouy-en-Josas
  • UMR 5091 CNRS Université Bordeaux 2 Institut François Magendie
  • Department of Biochemistry and Biophysics University of California San Francisco
  • BIOTEC Technical University of Dresden
  • Innopsys
  • Nikon B.V
  • Roper Scientific

Keywords: Imaging Optical and electron microscopy Cell and molecular biology Membrane transport